Granzyme B (GZMB) (NM_004131) Human Recombinant Protein

CAT#: TP306495

Purified recombinant protein of Human granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1) (GZMB), full length, with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20 µg

Product Images

Purified recombinant protein GZMB was analyzed by SDS-PAGE gel and Coomossie Blue Staining.

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  Recombinant Hu GZMB protein (TP306495) 100ng/mL was coated on plate and incubated at 4°C for overnight. After blocking with 1%BSA, 2x serially diluted primary antibody TA505931 (0.78 to 50ng/mL) and 10000x diluted HRP conjugated goat anti-mouse IgG were used for detection. Signal/Background ratio=37.5, EC50=98.91 ng/ml

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  Recombinant Hu GZMB protein (TP306495) 100ng/mL was coated on plate and incubated at 4°C for overnight. After blocking with 1%BSA, 2x serially diluted primary antibody TA505930 (0.78 to 50ng/mL) and 10000x diluted HRP conjugated goat anti-mouse IgG were used for detection. Signal/Background ratio=40.8, EC50=50.8 ng/ml

Specifications

Product Data

• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >RC206495 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MQPILLLLAFLLLPRADAGEIIGGHEAKPHSRPYMAYLMIWDQKSLKRCGGFLIQDDFVLTAAHCWGSSI
  NVTLGAHNIKEQEPTQQFIPVKRPIPHPAYNPKNFSNDIMLLQLERKAKRTRAVQPLRLPSNKAQVKPGQ
  TCSVAGWGQTAPLGKHSHTLQEVKMTVQEDRKCESDLRHYYDSTIELCVGDPEIKKTSFKGDSGGPLVCN
  KVAQGIVSYGRNNGMPPRACTKVSSFVHWIKKTMKRH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: Myc-DDK
• Predicted MW: 27.7 kDa
• Concentration: >0.05 µg/µL as determined by microplate Bradford method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_004122
• Locus ID: 3002
• UniProt ID: P10144, Q67BC3
• Cytogenetics: 14q12
• Refseq Size: 941
• Refseq ORF: 741
• Synonyms: C11; CCPI; CGL-1; CGL1; CSP-B; CSPB; CTLA1; CTSGL1; HLP; SECT
• Summary: This gene encodes a member of the granzyme subfamily of proteins, part of the peptidase S1 family of serine proteases. The encoded preproprotein is secreted by natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) and proteolytically processed to generate the active protease, which induces target cell apoptosis. This protein also processes cytokines and degrades extracellular matrix proteins, and these roles are implicated in chronic inflammation and wound healing. Expression of this gene may be elevated in human patients with cardiac fibrosis. [provided by RefSeq, Sep 2016]
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Allograft rejection, Autoimmune thyroid disease, Graft-versus-host disease, Natural killer cell mediated cytotoxicity, Type I diabetes mellitus