S100A9 (NM_002965) Human Recombinant Protein

CAT#: TP308250

Purified recombinant protein of human S100 calcium binding protein A9 (S100A9), with C-terminal MYC/DDK tag, secretory expressed in HEK293 cells, 20ug

Product Images

NK cells, CD16+ or CD16- monocytes were either untreated (white bar) or pre-treated with various stimuli (grey bar), including S100A9 (OriGene TP308250), and co-cultured with KM966-coated A549 cells. The increases in A549 cell lysis in treated vs. untreated effector cells are plotted. * p < 0.05; **** p < 0.0001; ns: not significant. Figure cited from Sci Rep, PMID: 27670158

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  Coomassie blue staining of purified S100A9 protein (Cat# TP308250). The protein was produced from HEK293T cells transfected with S100A9 cDNA clone (Cat# RC208250) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Species: Human
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >RC208250 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTCKMSQLERNIETIINTFHQYSVKLGHPDTLNQGEFKELVRKDLQNFLKKENKNEKVIEHIMEDLDTNA
  DKQLSFEEFIMLMARLTWASHEKMHEGDEGPGHHHKPGLGEGTP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-Myc/DDK
• Predicted MW: 13.1 kDa
• Concentration: >50 ug/mL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol
• Bioactivity: Cell treatment (PMID: 26933915)
  Cell treatment (PMID: 27670158)
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Note: For culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_002956
• Locus ID: 6280
• UniProt ID: P06702
• Cytogenetics: 1q21.3
• Refseq Size: 586
• Refseq ORF: 342
• Synonyms: 60B8AG; CAGB; CFAG; CGLB; L1AG; LIAG; MAC387; MIF; MRP14; NIF; P14
• Summary: The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and altered expression of this protein is associated with the disease cystic fibrosis. This antimicrobial protein exhibits antifungal and antibacterial activity. [provided by RefSeq, Nov 2014]