Estrogen Receptor 1 (ESR1) (NM_000125) Human Recombinant Protein

CAT#: TP313277

Recombinant protein of human estrogen receptor 1 (ESR1), transcript variant 1

Product Images

Coomassie blue staining of purified ESR1 protein (Cat# TP313277). The protein was produced from HEK293T cells transfected with ESR1 cDNA clone (Cat# RC213277) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Species: Human
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Recombinant protein was produced with TrueORF clone, RC213277. Click on the TrueORF clone link to view cDNA and protein sequences.
• Tag: C-Myc/DDK
• Predicted MW: 66 kDa
• Concentration: >50 ug/mL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol
• Bioactivity: Binding assay (PMID: 28842010)
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_000116
• Locus ID: 2099
• UniProt ID: P03372, G4XH65
• Cytogenetics: 6q25.1-q25.2
• Refseq Size: 6456
• Refseq ORF: 1785
• Synonyms: ER; Era; ESR; ESRA; ESTRR; NR3A1
• Summary: 'This gene encodes an estrogen receptor and ligand-activated transcription factor. The canonical protein contains an N-terminal ligand-independent transactivation domain, a central DNA binding domain, a hinge domain, and a C-terminal ligand-dependent transactivation domain. The protein localizes to the nucleus where it may form either a homodimer or a heterodimer with estrogen receptor 2. The protein encoded by this gene regulates the transcription of many estrogen-inducible genes that play a role in growth, metabolism, sexual development, gestation, and other reproductive functions and is expressed in many non-reproductive tissues. The receptor encoded by this gene plays a key role in breast cancer, endometrial cancer, and osteoporosis. This gene is reported to have dozens of transcript variants due to the use of alternate promoters and alternative splicing, however, the full-length nature of many of these variants remain uncertain. [provided by RefSeq, Jul 2020]'
• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors