HAO1 (NM_017545) Human Recombinant Protein

CAT#: TP316834

Recombinant protein of human hydroxyacid oxidase (glycolate oxidase) 1 (HAO1)

Product Images

Coomassie blue staining of purified HAO1 protein (Cat# TP316834). The protein was produced from HEK293T cells transfected with HAO1 cDNA clone (Cat# RC216834) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Species: Human
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Recombinant protein was produced with TrueORF clone, RC216834. Click on the TrueORF clone link to view cDNA and protein sequences.
• Tag: C-Myc/DDK
• Predicted MW: 40.7 kDa
• Concentration: >50 ug/mL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_060015
• Locus ID: 54363
• UniProt ID: Q9UJM8, A8K058
• Cytogenetics: 20p12.3
• Refseq Size: 1746
• Refseq ORF: 1110
• Synonyms: GOX; GOX1; HAOX1
• Summary: This gene is one of three related genes that have 2-hydroxyacid oxidase activity yet differ in encoded protein amino acid sequence, tissue expression and substrate preference. Subcellular location of the encoded protein is the peroxisome. Specifically, this gene is expressed primarily in liver and pancreas and the encoded protein is most active on glycolate, a two-carbon substrate. The protein is also active on 2-hydroxy fatty acids. The transcript detected at high levels in pancreas may represent an alternatively spliced form or the use of a multiple near-consensus upstream polyadenylation site. [provided by RefSeq, Jul 2008]
• Protein Pathways: Glyoxylate and dicarboxylate metabolism, Metabolic pathways