USP8 (NM_005154) Human Recombinant Protein

CAT#: TP316926

Recombinant protein of human ubiquitin specific peptidase 8 (USP8), transcript variant 1

Product Images

Coomassie blue staining of purified USP8 protein (Cat# TP316926). The protein was produced from HEK293T cells transfected with USP8 cDNA clone (Cat# RC216926) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Species: Human
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Recombinant protein was produced with TrueORF clone, RC216926. Click on the TrueORF clone link to view cDNA and protein sequences.
• Tag: C-Myc/DDK
• Predicted MW: 127.3 kDa
• Concentration: >50 ug/mL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_005145
• Locus ID: 9101
• UniProt ID: P40818, A0A024R5S4, A8K8N5
• Cytogenetics: 15q21.2
• Refseq Size: 4250
• Refseq ORF: 3354
• Synonyms: HumORF8; PITA4; SPG59; UBPY
• Summary: This gene encodes a protein that belongs to the ubiquitin-specific processing protease family of proteins. The encoded protein is thought to regulate the morphology of the endosome by ubiquitination of proteins on this organelle and is involved in cargo sorting and membrane trafficking at the early endosome stage. This protein is required for the cell to enter the S phase of the cell cycle and also functions as a positive regulator in the Hedgehog signaling pathway in development. Pseudogenes of this gene are present on chromosomes 2 and 6. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Endocytosis