T (NM_003181) Human Recombinant Protein

CAT#: TP318530

Recombinant protein of human T, brachyury homolog (mouse) (T)

Product Images

Coomassie blue staining of purified TBXT protein (Cat# TP318530). The protein was produced from HEK293T cells transfected with TBXT cDNA clone (Cat# RC218530) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Species: Human
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Recombinant protein was produced with TrueORF clone, RC218530. Click on the TrueORF clone link to view cDNA and protein sequences.
• Tag: C-Myc/DDK
• Predicted MW: 47.3 kDa
• Concentration: >50 ug/mL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_003172
• Locus ID: 6862
• UniProt ID: O15178
• Cytogenetics: 6q27
• Refseq Size: 2518
• Refseq ORF: 1308
• Synonyms: SAVA; T; TFT
• Summary: 'The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. Variation in this gene was associated with susceptibility to neural tube defects and chordoma. A mutation in this gene was found in a family with sacral agenesis with vertebral anomalies. [provided by RefSeq, Sep 2018]'
• Protein Families: ES Cell Differentiation/IPS, Transcription Factors