SMURF1 (NM_181349) Human Recombinant Protein

CAT#: TP319536

Recombinant protein of human SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant 2

Product Images

Coomassie blue staining of purified SMURF1 protein (Cat# TP319536). The protein was produced from HEK293T cells transfected with SMURF1 cDNA clone (Cat# RC219536) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Species: Human
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Recombinant protein was produced with TrueORF clone, RC219536. Click on the TrueORF clone link to view cDNA and protein sequences.
• Tag: C-Myc/DDK
• Predicted MW: 83.3 kDa
• Concentration: >50 ug/mL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_851994
• Locus ID: 57154
• UniProt ID: Q9HCE7
• Cytogenetics: 7q22.1
• Refseq Size: 5659
• Refseq ORF: 2193
• Synonyms: KIAA1625
• Summary: This gene encodes a ubiquitin ligase that is specific for receptor-regulated SMAD proteins in the bone morphogenetic protein (BMP) pathway. This protein plays a key roll in the regulation of cell motility, cell signalling, and cell polarity. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2010]
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis, TGF-beta signaling pathway, Ubiquitin mediated proteolysis