EEF1G (NM_001404) Human Recombinant Protein

CAT#: TP319578

Recombinant protein of human eukaryotic translation elongation factor 1 gamma (EEF1G)

Product Images

Biolayer interferometry assays (BLI) of a biotinylated HIV RTp66/p51-loaded or integrase-loaded biosensor with 60 nM of purifiedeEF1A (OriGene TP309697), eEF1B (OriGene TP321264), eEF1D (OriGene TP303202), eEF1G (OriGene TP319578) or BSA as analytes. Figure cited from PLoS Pathog, PMID: 26624286

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  Coomassie blue staining of purified EEF1G protein (Cat# TP319578). The protein was produced from HEK293T cells transfected with EEF1G cDNA clone (Cat# RC219578) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Species: Human
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Recombinant protein was produced with TrueORF clone, RC219578. Click on the TrueORF clone link to view cDNA and protein sequences.
• Tag: C-Myc/DDK
• Predicted MW: 49.9 kDa
• Concentration: >50 ug/mL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol
• Bioactivity: Biolayer interferometry (BLI) assay (PMID: 26624286)
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_001395
• Locus ID: 1937
• UniProt ID: P26641, Q53YD7
• Cytogenetics: 11q12.3
• Refseq Size: 1429
• Refseq ORF: 1311
• Synonyms: EF1G; GIG35
• Summary: 'This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit contains an N-terminal glutathione transferase domain, which may be involved in regulating the assembly of multisubunit complexes containing this elongation factor and aminoacyl-tRNA synthetases. [provided by RefSeq, Jul 2008]'