MIB1 (NM_020774) Human Recombinant Protein

CAT#: TP321377

Recombinant protein of human mindbomb homolog 1 (Drosophila) (MIB1)

Product Images

Coomassie blue staining of purified MIB1 protein (Cat# TP321377). The protein was produced from HEK293T cells transfected with MIB1 cDNA clone (Cat# RC221377) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Species: Human
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Recombinant protein was produced with TrueORF clone, RC221377. Click on the TrueORF clone link to view cDNA and protein sequences.
• Tag: C-Myc/DDK
• Predicted MW: 110 kDa
• Concentration: >50 ug/mL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol
• Bioactivity: In vitro ubiquitination assay (PMID: 27882347)
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_065825
• Locus ID: 57534
• UniProt ID: Q86YT6
• Cytogenetics: 18q11.2
• Refseq Size: 9308
• Refseq ORF: 3018
• Synonyms: DIP-1; DIP1; LVNC7; MIB; ZZANK2; ZZZ6
• Summary: This gene encodes a protein containing multiple ankyrin repeats and RING finger domains that functions as an E3 ubiquitin ligase. The encoded protein positively regulates Notch signaling by ubiquitinating the Notch receptors, thereby facilitating their endocytosis. This protein may also promote the ubiquitination and degradation of death-associated protein kinase 1 (DAPK1). [provided by RefSeq, Jun 2013]
• Protein Families: Druggable Genome