Mcm7 (NM_008568) Mouse Recombinant Protein

CAT#: TP510265

Purified recombinant protein of Mouse minichromosome maintenance complex component 7 (Mcm7), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Specifications

Product Data

• Species: Mouse
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  A DNA sequence from Mouse cDNA ORF Clone, MR210265, encoding Mouse full-length Mcm7.
• Tag: C-MYC/DDK
• Predicted MW: 81.2 kDa
• Concentration: >50 ug/mL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_032594
• Locus ID: 17220
• UniProt ID: Q61881, Q3U4T8
• Cytogenetics: 5 G2
• Refseq Size: 2396
• Refseq ORF: 2160
• Synonyms: AI747533; mCDC47; Mcmd7
• Summary: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage. [UniProtKB/Swiss-Prot Function]