G CSF (CSF3) (NM_000759) Human Recombinant Protein
CAT#: TP720002L
Recombinant protein of human colony stimulating factor 3 (granulocyte) (CSF3), transcript variant 1, produced in E. coli
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Specifications
Product Data | |
Species | Human |
Expression Host | E. coli |
Expression cDNA Clone or AA Sequence |
Thr31-Pro204
|
Tag | tag free |
Predicted MW | 18.8 kDa |
Concentration | lot specific |
Purity | >95% as determined by SDS-PAGE and Coomassie blue staining |
Buffer | Lyophilized from a 0.2 um filtered solution of 10mM HAc-NaAc, 150mM NaCl, 0.004% Tween 80, 5% Mannitol, pH 4.0. |
Bioactivity | ED50 is less than 0.1 ng/ml as calculated by the dose-dependent proliferation of murine NFS-60 indicator cells. Specific Activity of 6.0 x 107 IU/ mg. |
Endotoxin | < 0.1 EU per µg protein as determined by LAL test |
Storage | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
Stability | Stable for at least 6 months from date of receipt under proper storage and handling conditions. |
Reference Data | |
RefSeq | NP_000750 |
Locus ID | 1440 |
UniProt ID | P09919, Q8N4W3 |
Cytogenetics | 17q21.1 |
Synonyms | C17orf33; CSF3OS; GCSF |
Summary | This gene encodes a member of the IL-6 superfamily of cytokines. The encoded cytokine controls the production, differentiation, and function of granulocytes. Granulocytes are a type of white blood cell that are part of the innate immune response. A modified form of this protein is commonly administered to manage chemotherapy-induced neutropenia. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2020] |
Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein |
Protein Pathways | Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Jak-STAT signaling pathway |
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