IDE (NM_004969) Human Recombinant Protein

CAT#: TP721043

Purified recombinant protein of Human insulin-degrading enzyme (IDE), transcript variant 1

Specifications

Product Data

• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Met42-Leu1019
• Tag: C-His
• Predicted MW: 41.27 kDa
• Purity: >95% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: Supplied as a 0.2 um filtered solution of 20mM Tris-HCl, 150mM NaCl, 0.05%Brij35, 10% Glycerol, pH 7.5.
• Endotoxin: Endotoxin level is < 0.1 ng/µg of protein (< 1 EU/µg)
• Storage: Store at < -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
• Stability: Stable for at least 3 months from date of receipt under proper storage and handling conditions.

Reference Data

• RefSeq: NP_004960
• Locus ID: 3416
• UniProt ID: P14735
• Cytogenetics: 10q23.33
• Refseq Size: 3279
• Refseq ORF: 3057
• Synonyms: INSULYSIN
• Summary: This gene encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described but have not been experimentally verified.[provided by RefSeq, Sep 2009]
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Alzheimer's disease