Mesothelin (MSLN) Human Recombinant Protein

CAT#: TP721267

Biotinylated Human Mesothelin (E296-G580) Protein (C-His-Avi)

Product Images

Gel Shifting Assay: (1) Biotinylated Human MSLN Protein (C-His-Avi) and (2) Human MSLN Protein (C-His-Avi) on SDS-PAGE under reducing condition.

Specifications

Product Data

• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  E296-G580
• Tag: C-His-Avi
• Predicted MW: The protein has a predicted molecular weight of 35.8 kDa and migrates at approximately 35-45 kDa on SDS-PAGE with DTT-reduced conditions.
• Concentration: 25µg size is bottled at 0.2mg/mL concentration. 100 µg size is bottled at lot specific concentration.
• Purity: >90%
• Buffer: 1xPBS buffer, pH7.4
• Bioactivity: Positive
  
  The definition of the active protein (purified and biotinylated) is defined as the protein that can bind to its biological receptor/ligand. For conjugated protein, it is defined with its function to bind to the ScFv of the active CAR-transfected cells in flow cytometry test.
• Preparation: Affinity Ni-NTA
• Applications: ELISA
• Storage: An unopened vial can be stored at 4°C for 2 weeks or at -20°C and below for six months. This stock solution should be aliquoted and stored at ≤ -70°C to minimize the freeze/thaw cycles.
• Stability: 6 Months

Reference Data

• RefSeq: AAH09272.1
• Locus ID: 10232
• UniProt ID: Q13421