Her2 (ERBB2) Human Recombinant Protein

CAT#: TP721314

Human Her2 Protein (C-His)

Product Images

Human HER2 protein (C-His) is coated at 200ng/well. Anti-Her2 antibody can detect the Her2 protein. The ED50 is about 41.25 ng/mL. .

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  Human HER2 Protein (C-His) on SDS-PAGE under reducing condition P(+) and non-reducing condition P(-). The purity of this protein appears to be greater than 90% based on Coomassie-blue staining.

Specifications

Product Data

• Species: Human
• Expression Host: CHO
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Ser22-Thr652
• Tag: C-His
• Predicted MW: The protein has a predicted molecular weight of 71 kDa and migrates at approximately 80 kDa on SDS-PAGE with DTT-reduced conditions.
• Concentration: 25µg size is bottled at 0.2mg/mL concentration. 100 µg size is bottled at lot specific concentration.
• Purity: >90%
• Buffer: 1xPBS buffer, pH7.4
• Bioactivity: Positive
  
  The definition of the active protein (purified and biotinylated) is defined as the protein that can bind to its biological receptor/ligand. For conjugated protein, it is defined with its function to bind to the ScFv of the active CAR-transfected cells in flow cytometry test.
• Preparation: Affinity Ni-NTA
• Applications: ELISA
• Storage: An unopened vial can be stored at 4°C for 2 weeks or at -20°C and below for six months. This stock solution should be aliquoted and stored at ≤ -70°C to minimize the freeze/thaw cycles.
• Stability: 6 Months

Reference Data

• RefSeq: NP_004439.2
• Locus ID: 2064
• UniProt ID: P04626
• Summary: Human epithelial growth factor receptor 2 (HER2), also named ErbB2/Neu receptor, is a member of the epidermal growth factor receptor (EGFR; also known as ErbB) family of receptor tyrosine kinases. In human, this family are consisted of four members: HER1 (EGFR, ERBB1), HER2 (ERBB2), HER3 (ERBB3) and HER4 (ERBB4). The HER family proteins are type I transmembrane growth factor receptors that function to activate intracellular signaling pathways in response to extracellular signals. Their structure consists of an extracellular ligand binding domain, a transmembrane domain, and an intracellular tyrosine kinase domain. Unlike other members of the family, HER2 lacks ligand binding activity and its signaling function is engaged by its ligand-bound heterodimeric partners. Its expression has a close relationship with various tumors. Its overexpression is found in malignant tumors, such as breast, ovarian, gastric, and colorectal cancers.