Her2 (ERBB2) Human Recombinant Protein

CAT#: TP721318

APC Conjugated Human Her2 Protein (C-His)

Specifications

Product Data

• Species: Human
• Expression Host: CHO
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Ser22-Thr652
• Tag: C-His
• Predicted MW: The protein has a predicted molecular weight of 71 kDa and migrates at approximately 80 kDa on SDS-PAGE with DTT-reduced conditions before PE conjugation.
• Concentration: 25µg size is bottled at 0.1mg/mL concentration. 100 µg size is bottled at lot specific concentration.
• Purity: >90%
• Buffer: 1xPBS buffer, pH7.4, 0.09% NaN3 with a carrier protein
• Bioactivity: Positive
  
  The definition of the active protein (purified and biotinylated) is defined as the protein that can bind to its biological receptor/ligand. For conjugated protein, it is defined with its function to bind to the ScFv of the active CAR-transfected cells in flow cytometry test.
• Preparation: Affinity Ni-NTA
• Applications: FACS
• Storage: Unopened vial can be stored at 4°C for six months.
• Stability: 6 Months

Reference Data

• RefSeq: NP_004439.2
• Locus ID: 2064
• UniProt ID: P04626
• Summary: Human epithelial growth factor receptor 2 (HER2), also named ErbB2/Neu receptor, is a member of the epidermal growth factor receptor (EGFR; also known as ErbB) family of receptor tyrosine kinases. In human, this family are consisted of four members: HER1 (EGFR, ERBB1), HER2 (ERBB2), HER3 (ERBB3) and HER4 (ERBB4). The HER family proteins are type I transmembrane growth factor receptors that function to activate intracellular signaling pathways in response to extracellular signals. Their structure consists of an extracellular ligand binding domain, a transmembrane domain, and an intracellular tyrosine kinase domain. Unlike other members of the family, HER2 lacks ligand binding activity and its signaling function is engaged by its ligand-bound heterodimeric partners. Its expression has a close relationship with various tumors. Its overexpression is found in malignant tumors, such as breast, ovarian, gastric, and colorectal cancers.