TNF alpha (TNF) (NM_000594) Human Recombinant Protein

CAT#: TP723708

Purified recombinant protein of Human tumor necrosis factor (TNF)


  View other "TNF" proteins (11)

USD 205.00

5 Days*

Size
    • 10 ug

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Specifications

Product Data
Species Human
Expression Host E. coli
Expression cDNA Clone or AA Sequence
Human TNF-alpha;, the region of Val77-Leu233, from gene Accession# NM_000594
Tag Tag Free
Predicted MW 17 kDa
Concentration Protein formulated and vialed at >50 ug/mL in specified buffer
Purity Purity is >98%, as determined by Coomassie stained SDS-PAGE.
Buffer 10mM NaH2PO4, 150mM NaCl, pH7.2
Bioactivity The ED50 is 0.020-0.050 ng/ml, corresponding to a specific activity of 5-2 X10^7 units/mg, determined by a dose dependent stimulation of L929 cells treated with actinomycin D.
Endotoxin Less than 0.01 ng per ug protein as determined by the LAL method
Storage Store at -80°C.
Stability Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to 6 months, or at -70°C or below until the expiration date. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Reference Data
RefSeq NP_000585
Locus ID 7124
UniProt ID P01375, Q5STB3
Cytogenetics 6p21.33
Refseq Size 1686
Refseq ORF 699
Synonyms DIF; TNF-alpha; TNFA; TNFSF2; TNLG1F
Summary 'This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, psoriasis, rheumatoid arthritis ankylosing spondylitis, tuberculosis, autosomal dominant polycystic kidney disease, and cancer. Mutations in this gene affect susceptibility to cerebral malaria, septic shock, and Alzheimer disease. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Aug 2020]'
Protein Families Druggable Genome, Secreted Protein, Transcription Factors, Transmembrane
Protein Pathways Adipocytokine signaling pathway, Allograft rejection, Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Asthma, Cytokine-cytokine receptor interaction, Dilated cardiomyopathy, Fc epsilon RI signaling pathway, Graft-versus-host disease, Hematopoietic cell lineage, Hypertrophic cardiomyopathy (HCM), MAPK signaling pathway, Natural killer cell mediated cytotoxicity, NOD-like receptor signaling pathway, RIG-I-like receptor signaling pathway, Systemic lupus erythematosus, T cell receptor signaling pathway, TGF-beta signaling pathway, Toll-like receptor signaling pathway, Type I diabetes mellitus, Type II diabetes mellitus

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.