Il27 (NM_145636) Mouse Recombinant Protein

CAT#: TP723758

Purified recombinant protein of Mouse interleukin 27 (Il27)

Product Images

Specifications

Product Data

• Species: Mouse
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Secreted mIL-27 protein consists of mouse EBI3 (Tyr19-Pro228) from gene Accession# NM_015766, and mouse p28, the region of Phe29-Ser234) from gene Accession (NM_145636), linked via GS bridge
• Tag: Tag Free
• Predicted MW: 49 kDa
• Concentration: Protein formulated and vialed at >50 ug/mL in specified buffer
• Purity: Purity is >95%, as determined by Coomassie stained SDS-PAGE.
• Buffer: 20 mM NaH2PO4, pH6, 0.60 M NaCl
• Bioactivity: The ED50 is 5 - 10 ng/ml, corresponding to a specific activity of 1.0 - 2.0 x 10^5 units/mg, determined by inhibition of IL-2 production induced by mouse IL-27 in mouse splenocytes activated with anti-CD3 and anti-CD28 antibodies.
• Endotoxin: Less than 0.01 ng per µg protein as determined by the LAL method
• Storage: Store at -80°C.
• Stability: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to 6 months, or at -70°C or below until the expiration date. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.

Reference Data

• RefSeq: NP_663611
• Locus ID: 246779
• UniProt ID: Q8K3I6
• Cytogenetics: 7 F3
• Refseq Size: 705
• Refseq ORF: 702
• Synonyms: IL-27; IL-27-A; IL-27p28; IL27-A; Il30; p28