IL3 (NM_000588) Human Recombinant Protein

CAT#: TP723764

Purified recombinant protein of Human interleukin 3 (colony-stimulating factor, multiple) (IL3)

Specifications

Product Data

• Species: Human
• Expression Host: Sf9
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Human IL-3, the region of Ala20-Phe152, from gene Accession# NM_000588
• Tag: Tag Free
• Predicted MW: 15 kDa
• Concentration: Protein formulated and vialed at >50 ug/mL in specified buffer
• Purity: >98%, as determined by Coomassie stained SDS-PAGE.
• Buffer: 20 mM NaH2PO4, 0.30 M NaCl, pH6.0
• Bioactivity: The ED50 is 0.10 - 0.30 ng/ml, corresponding to a specific activity of 0.33 - 1.0 x 10^7 units/mg, determined by the dose dependent stimulation of TF-1 cell proliferation assay. Under this assay the bioactivity is equivalent to competit
• Endotoxin: Less than 0.01 ng per µg protein as determined by the LAL method
• Storage: Store at -80°C.
• Stability: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to 6 months, or at -70°C or below until the expiration date. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.

Reference Data

• RefSeq: NP_000579
• Locus ID: 3562
• UniProt ID: P08700
• Cytogenetics: 5q31.1
• Refseq Size: 924
• Refseq ORF: 456
• Synonyms: IL-3; MCGF; MULTI-CSF
• Summary: 'The protein encoded by this gene is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders. [provided by RefSeq, Jul 2008]'
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Apoptosis, Asthma, Cytokine-cytokine receptor interaction, Fc epsilon RI signaling pathway, Hematopoietic cell lineage, Jak-STAT signaling pathway