TSLP (NM_033035) Human Recombinant Protein

CAT#: TP723796

Purified recombinant protein of Human thymic stromal lymphopoietin (TSLP), transcript variant 1

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Human TSLP,the rehion of Met- Tyr29-Gln159, from gene accession# NM_033035.4
• Tag: Tag Free
• Predicted MW: 15 kDa
• Concentration: Protein formulated and vialed at >50 ug/mL in specified buffer
• Purity: >98%, as determined by Coomassie stained SDS-PAGE.
• Buffer: 20 mM NaHPO4, pH7.2, 0.15 M NaCl.
• Bioactivity: The ED50 is 1 - 5 ng/ml, corresponding to a specific activity of 0.2 - 1 x 10^6 units/mg, determined by the dose dependent stimulation of mouse BaF3-human IL-7a and human TSLPR cotransfectant cell proliferation.
• Endotoxin: Less than 0.01 ng per µg protein as determined by the LAL method
• Storage: Store at -80°C.
• Stability: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to 6 months, or at -70°C or below until the expiration date. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.

Reference Data

• RefSeq: NP_149024
• Locus ID: 85480
• UniProt ID: Q969D9
• Cytogenetics: 5q22.1
• Refseq Size: 2652
• Refseq ORF: 477
• Synonyms: thymic stromal lymphopoietin
• Summary: This gene encodes a hemopoietic cytokine proposed to signal through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the IL-7R alpha chain. It mainly impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of CD11c(+) dendritic cells. The protein promotes T helper type 2 (TH2) cell responses that are associated with immunity in various inflammatory diseases, including asthma, allergic inflammation and chronic obstructive pulmonary disease. The protein is therefore considered a potential therapeutic target for the treatment of such diseases. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2012]
• Protein Families: Druggable Genome
• Protein Pathways: Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway