PCSK9 (NM_174936) Human Recombinant Protein

CAT#: TP723879

Purified recombinant protein of Human proprotein convertase subtilisin/kexin type 9 (PCSK9)

Specifications

Product Data

• Species: Human
• Expression Host: CHO
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Human PCSK9, the region of Arg29-Gln692, from gene Accession# NM_174936
• Tag: C-His
• Predicted MW: 73 kDa
• Concentration: Protein formulated and vialed at >50 ug/mL in specified buffer
• Purity: >90%, as determined by Coomassie stained SDS-PAGE.
• Buffer: 25 mM sodium Acetate, 150 mM NaCl, pH5.0
• Bioactivity: Human PCSK9 binds human LDLR. The amount of human LDLR bound in assay conditions determined by ELISA.
• Endotoxin: Less than 1 EU per µg protein as determine by the LAL method
• Storage: Store at -80°C.
• Stability: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to 6 months, or at -70°C or below until the expiration date. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.

Reference Data

• RefSeq: NP_777596
• Locus ID: 255738
• UniProt ID: Q8NBP7
• Cytogenetics: 1p32.3
• Refseq Size: 3636
• Refseq ORF: 2076
• Synonyms: FH3; HCHOLA3; LDLCQ1; NARC-1; NARC1; PC9
• Summary: This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an autocatalytic processing event with its prosegment in the ER and is constitutively secreted as an inactive protease into the extracellular matrix and trans-Golgi network. It is expressed in liver, intestine and kidney tissues and escorts specific receptors for lysosomal degradation. It plays a role in cholesterol and fatty acid metabolism. Mutations in this gene have been associated with autosomal dominant familial hypercholesterolemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]
• Protein Families: Secreted Protein