SARS-CoV-2 Spike S1 Recombinant Protein with His and Avi tag

CAT#: TP723916

SARS-CoV-2 Spike S1 Recombinant Protein with His and Avi tag

Product Images

The purity of SARS-COV-2 Spike S1 Protein with His and Avi tag (Cat.TP723916) was greater than 95% as determined by SEC-HPLC.

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  Immobilized Human ACE2 on COOH Chip, can bind SARS-COV-2 Spike S1 with an affinity constant of 24.5 nM as determined in a SPR assay (Nicoya OpenSPR).

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  Immobilized Recombinant SARS-CoV-2 Spike S1 at 2μg/mL (100 μL/well) can bind recombinant Human ACE2 with a linear range of 0.15-6.85 ng/mL.

Specifications

Product Data

• Species: SARS-CoV-2
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  QCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPRR
• Tag: C-6xHis/AVI
• Purity: >95% by SDS-PAGE.
• Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
• Endotoxin: < 1.0 EU/μg of the protein by LAL method.
• Protein Description: Recombinant 2019-nCoV Spike S1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln14-Arg683) of 2019-ncov Spike S1 (Accession #YP_009724390.1) fused with a 6×His tag and Avi at the C-terminus.
• Storage: Store at -80°C.
• Stability: 3 months

Reference Data

• Locus ID: 43740568