SIRPA Human Recombinant Protein

CAT#: TP723932

Human SIRP alpha Protein, hFc-His Tag

Product Images

Figure 1. Human SIRPα, hFc-His Tag on SDS-PAGE under reducing condition.

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  Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human CD47, mFc-His tagged protein (TP723931) can bind its native ligand Human SIRPα, hFc-His tagged protein (TP723932) in a linear range of 3.3-26.37 ng/ml.

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  Figure 3. A: Human SIRPα, hFc-His tagged protein without freeze-thaw treatment. B: Human SIRPα, hFc-His tagged protein after one freeze-thaw cycle. C: Human SIRPα, hFc-His tagged protein after three freeze-thaw cycles. D: Human SIRPα, hFc-His tagged protein after five freeze-thaw cycles.

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  Figure 4. Lane 1: Human SIRPα, hFc-His tagged protein without freeze-thaw treatment, Lane 2: Human SIRPα, hFc-His tagged protein after one freeze-thaw cycle, Lane 3: Human SIRPα, hFc-His tagged protein after three freeze-thaw cycles, Lane 4: Human SIRPα, hFc-His tagged protein after five freeze-thaw cycles.

Specifications

Product Data

• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  SIRPα（Glu31-Arg370）+hFc（Glu99-Ala330）+6×His tag
• Tag: C-Human Fc and 6×His
• Predicted MW: 70-98 kDa
• Purity: > 95%
• Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.
• Reconstitution: Reconstitute with deionized water
• Preparation: Affinity purification
• Applications: ELISA
• Storage: Store at -80°C for 12 months (Avoid repeated freezing and thawing)
• Stability: 12 months from date of despatch

Reference Data

• Locus ID: 140885