TdT (DNTT) (NM_001017520) Human Recombinant Protein

CAT#: TP761324

Purified recombinant protein of Human deoxynucleotidyltransferase, terminal (DNTT), transcript variant 2, full length, with N-terminal GST and C-terminal His tag, expressed in E. coli, 50ug

Product Images

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  A DNA sequence encoding human full-length DNTT
• Tag: N-GST and C-His
• Predicted MW: 84.2 kDa
• Concentration: >50 ug/mL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25mM Tris, pH8.0,150mM NaCl,10% glycerol,1% Sarkosyl.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_001017520
• Locus ID: 1791
• UniProt ID: P04053
• Cytogenetics: 10q24.1
• Refseq Size: 2068
• Refseq ORF: 1527
• Synonyms: TDT
• Summary: 'This gene is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. In vivo, the encoded protein is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq, Jul 2008]'
• Protein Pathways: Hematopoietic cell lineage, Non-homologous end-joining