LIM kinase 2 (LIMK2) (NM_001031801) Human Recombinant Protein
CAT#: TP761350
Purified recombinant protein of Human LIM domain kinase 2 (LIMK2), transcript variant 1, full length, with N-terminal GST and C-terminal His tag, expressed in E. coli, 50ug
Specifications
Product Data | |
Species | Human |
Expression Host | E. coli |
Expression cDNA Clone or AA Sequence |
A DNA sequence encoding human full-length LIMK2
|
Tag | N-GST and C-His |
Predicted MW | 103.7 kDa |
Concentration | >50 ug/mL as determined by microplate BCA method |
Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
Buffer | 25mM Tris, pH8.0,150mM NaCl,10% glycerol,1% Sarkosyl. |
Storage | Store at -80°C. |
Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
Reference Data | |
RefSeq | NP_001026971 |
Locus ID | 3985 |
UniProt ID | P53671 |
Cytogenetics | 22q12.2 |
Refseq Size | 2914 |
Refseq ORF | 2058 |
Summary | 'There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]' |
Protein Families | Druggable Genome, Protein Kinase |
Protein Pathways | Axon guidance, Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton |
Documents
FAQs |
Resources
Recombinant Protein Resources |
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
LC413829 | LIMK2 HEK293T cell transient overexpression lysate (as WB positive control) |
USD 187.00 |
|
LC422193 | LIMK2 HEK293T cell transient overexpression lysate (as WB positive control) |
USD 121.00 |
|
LY413829 | Transient overexpression lysate of LIM domain kinase 2 (LIMK2), transcript variant 2b |
USD 605.00 |
|
LY422193 | Transient overexpression lysate of LIM domain kinase 2 (LIMK2), transcript variant 1 |
USD 396.00 |
|
PH320400 | LIMK2 MS Standard C13 and N15-labeled recombinant protein (NP_057952) |
USD 2,055.00 |
|
TP320400 | Recombinant protein of human LIM domain kinase 2 (LIMK2), transcript variant 2b |
USD 788.00 |
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