Neutrophil Elastase (ELANE) (NM_001972) Human Recombinant Protein

CAT#: TP761570

Purified recombinant protein ofHuman elastase, neutrophil expressed (ELANE), full length, with N-terminal GST and C-terminal HIS tag, expressed in E. coli, 50ug

Product Images

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  A DNA sequence encoding human full-length ELANE
• Tag: N-GST and C-His
• Predicted MW: 53.5 kDa
• Concentration: >50 ug/mL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25mM Tris, pH8.0,150mM NaCl,10% glycerol,1%Sarkosyl.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_001963
• Locus ID: 1991
• UniProt ID: P08246
• Cytogenetics: 19p13.3
• Refseq Size: 938
• Refseq ORF: 801
• Synonyms: ELA2; GE; HLE; HNE; NE; PMN-E; SCN1
• Summary: 'Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. The enzyme may play a role in degenerative and inflammatory diseases through proteolysis of collagen-IV and elastin. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is present in a gene cluster on chromosome 19. [provided by RefSeq, Jan 2016]'
• Protein Families: Protease, Transmembrane
• Protein Pathways: Systemic lupus erythematosus