MIR1244-3 Human MicroRNA Expression Plasmid (MI0015975)

CAT#: SC401260

Expression Plasmid of Human microRNA MIR1244-3

Specifications

Product Data

• Species: Human
• Vector: pCMV-MIR
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Precursor:

  AUCUUAUUCCGAGCAUUCCAGUAACUUUUUUGUGUAUGUACUUAGCUGUACUAUAAGUAGUUGGUUUGUAUGAGAUGGUUAAAAA
  Mature Sequence:

  AAGUAGUUGGUUUGUAUGAGAUGGUU
  Full insert

  >MI0015975 CCAACCCAAACCATGAGAATTTGCAACAGGGGAGGAAAAAAGAACCAAAACTTCCAAGGCCCTGCTTTTT
  TTCTTAAAAGTACTTTAAAAAGGAAATTTGTTTGTATTTTTTATTTACATTTTATATTTTTGTACATATT
  GTTAGGGTCAGCCATTTTTAATGATCTCGGATGACCAAACCAGCCTTCGGAGCGTTCTCTGTCCTACTTC
  TGACTTTACTTGTGGTGTGACCATGTTCATTATAATCTCAAAGGAGAAAAAAAACCTTGTAAAAAAAGCA
  AAAATGACAACAGAAAAACAATCTTATTCCGAGCATTCCAGTAACTTTTTTGTGTATGTACTTAGCTGTA
  CTATAAGTAGTTGGTTTGTATGAGATGGTTAAAAAGGCCAAAGATAAAAGGTTTCTTTTTTTTTCCTTTT
  TTGTCTATGAAGTTGCTGTTTATTTTTTTTGGCCTGTTTGATGTATGTGTGAAACAATGTTGTCCAACAA
  TAAACAGGAATTTTATTTTGCTGAGTTGTTCTAACAAAGCTGTCTCAAGCCTGGTTTTTCTGTTTCAGTT
  TCTTCAGACCTTCCAGGGCACAAGGATAGGAGGGGGAGGATCCTGGGGACAGTGGGTTGACATTTTGGGA
  GGCAGTTGGATGTGTCCTGTGATGGGCAGCAGAGTCTGGTGGGTGGGCAGGGACT
• OTI Disclaimer: All miRNA clones were sequenced to ensure that the pre-mir sequences match reference sequence in miRBase. The flanking sequence of a miRNA clone may differ from the NCBI reference with respect to biological polymorphisms. This should not affect the function of the mature miRNA.
• Product Components:
  • 1 x 10 ug vial miRNA plasmid lyophilized in a 2-D bar-coded Matrix tube
  • 1 x 100 pmol VP1.5 primer
  • 1 x 100 pmol XL39 primer
• Also Contains: Both mature and minor (star) miRNA can be over expressed from the construct.

Reference Data

• RefSeq: MI0015975
• Synonyms: MIR1244 MIRN1244 hsa-mir-1244 hsa-mir-1244-1
• Locus ID: 100302285