RECQ1 (RECQL) Human shRNA Lentiviral Particle (Locus ID 5965)

CAT#: TL302045V

RECQL - Human shRNA lentiviral particles (4 unique 29mer target-specific shRNA, 1 scramble control), 0.5 ml each, >10^7 TU/ml.

Product Images

GFP signal was observed under microscope at 48 hours after transduction of TL302045A virus into HEK293 cells. TL302045A virus was prepared using lenti-shRNA TL302045A and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL302045B virus into HEK293 cells. TL302045B virus was prepared using lenti-shRNA TL302045B and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL302045C virus into HEK293 cells. TL302045C virus was prepared using lenti-shRNA TL302045C and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL302045D virus into HEK293 cells. TL302045D virus was prepared using lenti-shRNA TL302045D and TR30037 packaging kit.

Specifications

Product Data

• Locus ID: 5965
• Synonyms: RecQ1; RECQL1
• Vector: pGFP-C-shLenti
• Format: Lentiviral particles
• RefSeq: NM_002907, NM_032941, BC001052
• Summary: 'The protein encoded by this gene is a member of the RecQ DNA helicase family. DNA helicases are enzymes involved in various types of DNA repair, including mismatch repair, nucleotide excision repair and direct repair. The encoded protein is involved in the processing of Holliday junctions, the suppression of sister chromatid exchanges, telomere maintenance, and is required for genotoxic stress resistance. Defects in this gene have been associated with several types of cancer. [provided by RefSeq, Jan 2017]'
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, align it with our published shRNA design sequences. If these do not align, please utilize our custom shRNA service
• Performance Guaranteed: OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
  
  For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).