Prion protein PrP (PRNP) Human shRNA Lentiviral Particle (Locus ID 5621)

CAT#: TL302273V

PRNP - Human shRNA lentiviral particles (4 unique 29mer target-specific shRNA, 1 scramble control), 0.5 ml each, >10^7 TU/ml.

Product Images

GFP signal was observed under microscope at 48 hours after transduction of TL302273A virus into HEK293 cells. TL302273A virus was prepared using lenti-shRNA TL302273A and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL302273B virus into HEK293 cells. TL302273B virus was prepared using lenti-shRNA TL302273B and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL302273B virus into HEK293 cells. TL302273B virus was prepared using lenti-shRNA TL302273B and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of [TL302273C] virus into HEK293 cells. [TL302273C] virus was prepared using lenti-shRNA [TL302273C] and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of [TL302273C] virus into HEK293 cells. [TL302273C] virus was prepared using lenti-shRNA [TL302273C] and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of [TL302273D] virus into HEK293 cells. [TL302273D] virus was prepared using lenti-shRNA [TL302273D] and TR30037 packaging kit.

• GFP signal was observed under microscope at 48 hours after transduction of [TL302273D] virus into HEK293 cells. [TL302273D] virus was prepared using lenti-shRNA [TL302273D] and TR30037 packaging kit.

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Specifications

Product Data

• Locus ID: 5621
• Synonyms: AltPrP; ASCR; CD230; CJD; GSS; KURU; p27-30; PRIP; PrP; PrP27-30; PrP33-35C; PrPc
• Vector: pGFP-C-shLenti
• Format: Lentiviral particles
• RefSeq: NM_000311, NM_001080121, NM_001080122, NM_001080123, NM_001271561, NM_183079, NM_001080123.1, NM_001080123.2, NM_000311.1, NM_000311.2, NM_000311.3, NM_000311.4, NM_183079.1, NM_183079.2, NM_183079.3, NM_001080122.1, NM_001080122.2, NM_001080121.1, NM_001080121.2, NM_001271561.1, NM_001271561.2, BC012844, BC012844.1, BC022532, BC022532.1, BC016809, NM_001271561.3, NM_183079.4, NM_001080122.3, NM_001080121.3, NM_001080123.3, NM_000311.5
• UniProt ID: P04156
• Summary: The protein encoded by this gene is a membrane glycosylphosphatidylinositol-anchored glycoprotein that tends to aggregate into rod-like structures. The encoded protein contains a highly unstable region of five tandem octapeptide repeats. This gene is found on chromosome 20, approximately 20 kbp upstream of a gene which encodes a biochemically and structurally similar protein to the one encoded by this gene. Mutations in the repeat region as well as elsewhere in this gene have been associated with Creutzfeldt-Jakob disease, fatal familial insomnia, Gerstmann-Straussler disease, Huntington disease-like 1, and kuru. An overlapping open reading frame has been found for this gene that encodes a smaller, structurally unrelated protein, AltPrp. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
• Performance Guaranteed: OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
  
  For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).