MAEL Human shRNA Lentiviral Particle (Locus ID 84944)

CAT#: TL303387V

MAEL - Human shRNA lentiviral particles (4 unique 29mer target-specific shRNA, 1 scramble control), 0.5 ml each, >10^7 TU/ml.

Product Images

GFP signal was observed under microscope at 48 hours after transduction of TL303387A virus into HEK293 cells. TL303387A virus was prepared using lenti-shRNA TL303387A and TR30037 packaging kit.

• 

  GFP signal was observed under microscope at 48 hours after transduction of TL303387B virus into HEK293 cells. TL303387B virus was prepared using lenti-shRNA TL303387B and TR30037 packaging kit.

• 

  GFP signal was observed under microscope at 48 hours after transduction of [TL303387C] virus into HEK293 cells. [TL303387C] virus was prepared using lenti-shRNA [TL303387C] and TR30037 packaging kit.

• 

  GFP signal was observed under microscope at 48 hours after transduction of [TL303387D] virus into HEK293 cells. [TL303387D] virus was prepared using lenti-shRNA [TL303387D] and TR30037 packaging kit.

Specifications

Product Data

• Locus ID: 84944
• Synonyms: CT128; SPATA35
• Vector: pGFP-C-shLenti
• Format: Lentiviral particles
• RefSeq: NM_001286377, NM_001286378, NM_032858, NM_032858.1, NM_032858.2, NM_001286378.1, NM_001286377.1, BC028595, BC028595.2, BC034310, NM_032858.3
• UniProt ID: Q96JY0
• Summary: Plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Its association with piP-bodies suggests a participation in the secondary piRNAs metabolic process. Required for the localization of germ-cell factors to the meiotic nuage (By similarity).[UniProtKB/Swiss-Prot Function]
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
• Performance Guaranteed: OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
  
  For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).