GPCR 2037 (GPR151) Human shRNA Lentiviral Particle (Locus ID 134391)

CAT#: TL304247V

LOC134391 - Human shRNA lentiviral particles (4 unique 29mer target-specific shRNA, 1 scramble control), 0.5 ml each, >10^7 TU/ml.

Product Images

GFP signal was observed under microscope at 48 hours after transduction of TL304247A virus into HEK293 cells. TL304247A virus was prepared using lenti-shRNA TL304247A and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL304247B virus into HEK293 cells. TL304247B virus was prepared using lenti-shRNA TL304247B and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of [TL304247C] virus into HEK293 cells. [TL304247C] virus was prepared using lenti-shRNA [TL304247C] and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of [TL304247D] virus into HEK293 cells. [TL304247D] virus was prepared using lenti-shRNA [TL304247D] and TR30037 packaging kit.

Specifications

Product Data

• Locus ID: 134391
• Synonyms: GALR4; GALRL; GPCR; GPCR-2037; PGR7
• Vector: pGFP-C-shLenti
• Format: Lentiviral particles
• RefSeq: NM_194251, NM_194251.1, NM_194251.2, BC140430, BC156567, NM_194251.3
• UniProt ID: Q8TDV0
• Summary: This gene encodes an orphan member of the class A rhodopsin-like family of G-protein-coupled receptors (GPCRs). Within the rhodopsin-like family, this gene is a member of the SOG subfamily that includes somatostatin, opioid, galanin, and kisspeptin receptors. The orthologous mouse gene has a restricted pattern of neuronal expression which is induced following nerve injury. All GPCRs have a transmembrane domain that includes seven transmembrane alpha-helices. A general feature of GPCR signaling is the agonist-induced conformational change in the receptor, leading to activation of the heterotrimeric G protein. The activated G protein then binds to and activates numerous downstream effector proteins, which generate second messengers that mediate a broad range of cellular and physiological processes. [provided by RefSeq, Jul 2017]
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
• Performance Guaranteed: OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
  
  For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).