CD47 Human shRNA Lentiviral Particle (Locus ID 961)

CAT#: TL305509V

CD47 - Human shRNA lentiviral particles (4 unique 29mer target-specific shRNA, 1 scramble control), 0.5 ml each, >10^7 TU/ml.

Product Images

GFP signal was observed under microscope at 48 hours after transduction of TL305509A virus into HEK293 cells. TL305509A virus was prepared using lenti-shRNA TL305509A and TR30037 packaging kit.

• 

  GFP signal was observed under microscope at 48 hours after transduction of TL305509B virus into HEK293 cells. TL305509B virus was prepared using lenti-shRNA TL305509B and TR30037 packaging kit.

• 

  GFP signal was observed under microscope at 48 hours after transduction of TL305509C virus into HEK293 cells. TL305509C virus was prepared using lenti-shRNA TL305509C and TR30037 packaging kit.

• 

  GFP signal was observed under microscope at 48 hours after transduction of TL305509D virus into HEK293 cells. TL305509D virus was prepared using lenti-shRNA TL305509D and TR30037 packaging kit.

Specifications

Product Data

• Locus ID: 961
• Synonyms: IAP; MER6; OA3
• Vector: pGFP-C-shLenti
• Format: Lentiviral particles
• RefSeq: NM_001025079, NM_001025080, NM_001777, NM_198793, BC037306, BC010016, BC012884, BC042889, BC045593, BC053959
• Summary: This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2010]
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, align it with our published shRNA design sequences. If these do not align, please utilize our custom shRNA service
• Performance Guaranteed: OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
  
  For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).