ARID1A Human shRNA Lentiviral Particle (Locus ID 8289)

CAT#: TL306603V

ARID1A - Human shRNA lentiviral particles (4 unique 29mer target-specific shRNA, 1 scramble control), 0.5 ml each, >10^7 TU/ml.

Product Images

GFP signal was observed under microscope at 48 hours after transduction of TL306603A virus into HEK293 cells. TL306603A virus was prepared using lenti-shRNA TL306603A and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL306603B virus into HEK293 cells. TL306603B virus was prepared using lenti-shRNA TL306603B and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL306603C virus into HEK293 cells. TL306603C virus was prepared using lenti-shRNA TL306603C and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL306603D virus into HEK293 cells. TL306603D virus was prepared using lenti-shRNA TL306603D and TR30037 packaging kit.

Specifications

Product Data

• Locus ID: 8289
• Synonyms: B120; BAF250; BAF250a; BM029; C1orf4; CSS2; ELD; hELD; hOSA1; MRD14; OSA1; P270; SMARCF1
• Vector: pGFP-C-shLenti
• Format: Lentiviral particles
• RefSeq: NM_006015, NM_018450, NM_139135, BC112895
• Summary: This gene encodes a member of the SWI/SNF family, whose members have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. It possesses at least two conserved domains that could be important for its function. First, it has a DNA-binding domain that can specifically bind an AT-rich DNA sequence known to be recognized by a SNF/SWI complex at the beta-globin locus. Second, the C-terminus of the protein can stimulate glucocorticoid receptor-dependent transcriptional activation. It is thought that the protein encoded by this gene confers specificity to the SNF/SWI complex and may recruit the complex to its targets through either protein-DNA or protein-protein interactions. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, align it with our published shRNA design sequences. If these do not align, please utilize our custom shRNA service
• Performance Guaranteed: OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
  
  For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).