YY1 Human shRNA Lentiviral Particle (Locus ID 7528)

CAT#: TL308320V

YY1 - Human shRNA lentiviral particles (4 unique 29mer target-specific shRNA, 1 scramble control), 0.5 ml each, >10^7 TU/ml.

Product Images

GFP signal was observed under microscope at 48 hours after transduction of TL308320A virus into HEK293 cells. TL308320A virus was prepared using lenti-shRNA TL308320A and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL308320B virus into HEK293 cells. TL308320B virus was prepared using lenti-shRNA TL308320B and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL308320D virus into HEK293 cells. TL308320D virus was prepared using lenti-shRNA TL308320D and TR30037 packaging kit.

Specifications

Product Data

• Locus ID: 7528
• Synonyms: DELTA; GADEVS; INO80S; NF-E1; UCRBP; YIN-YANG-1
• Vector: pGFP-C-shLenti
• Format: Lentiviral particles
• RefSeq: NM_003403, BC037308, BC014138, BC020324, BC065366
• Summary: 'YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1. [provided by RefSeq, Jul 2008]'
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, align it with our published shRNA design sequences. If these do not align, please utilize our custom shRNA service
• Performance Guaranteed: OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
  
  For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).