BRG1 (SMARCA4) Human shRNA Lentiviral Particle (Locus ID 6597)

CAT#: TL309249V

SMARCA4 - Human shRNA lentiviral particles (4 unique 29mer target-specific shRNA, 1 scramble control), 0.5 ml each, >10^7 TU/ml.

Product Images

GFP signal was observed under microscope at 48 hours after transduction of TL309249B virus into HEK293 cells. TL309249B virus was prepared using lenti-shRNA TL309249B and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of [TL309249C] virus into HEK293 cells. [TL309249C] virus was prepared using lenti-shRNA [TL309249C] and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of [TL309249D] virus into HEK293 cells. [TL309249D] virus was prepared using lenti-shRNA [TL309249D] and TR30037 packaging kit.

Specifications

Product Data

• Locus ID: 6597
• Synonyms: BAF190; BAF190A; BRG1; CSS4; hSNF2b; MRD16; RTPS2; SNF2; SNF2-beta; SNF2L4; SNF2LB; SWI2
• Vector: pGFP-C-shLenti
• Format: Lentiviral particles
• RefSeq: NM_001128844, NM_001128845, NM_001128846, NM_001128847, NM_001128848, NM_001128849, NM_003072, NM_003072.1, NM_003072.2, NM_003072.3, NM_001128848.1, NM_001128847.1, NM_001128846.1, NM_001128845.1, NM_001128844.1, NM_001128849.1, BC007302, BC035916, BC136644, BC139917, BC139924, BC150298, BC151849, NM_001128847.4, NM_001128846.2, NM_001128848.2, NM_001128849.3, NM_001128845.2, NM_003072.5, NM_001128844.3
• UniProt ID: P51532
• Summary: The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
• Performance Guaranteed: OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
  
  For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).