PLAGL1 Human shRNA Lentiviral Particle (Locus ID 5325)

CAT#: TL310381V

PLAGL1 - Human shRNA lentiviral particles (4 unique 29mer target-specific shRNA, 1 scramble control), 0.5 ml each, >10^7 TU/ml.

Specifications

Product Data

• Locus ID: 5325
• Synonyms: LOT1; ZAC; ZAC1
• Vector: pGFP-C-shLenti
• Format: Lentiviral particles
• RefSeq: NM_001080951, NM_001080952, NM_001080953, NM_001080954, NM_001080955, NM_001080956, NM_001289037, NM_001289038, NM_001289039, NM_001289040, NM_001289041, NM_001289042, NM_001289043, NM_001289044, NM_001289045, NM_001289046, NM_001289047, NM_001289048, NM_001289049, NM_001317156, NM_001317157, NM_001317158, NM_001317159, NM_001317160, NM_001317161, NM_001317162, NM_002656, NM_006718, BC109086, BC074814, BC109085
• Summary: 'This gene encodes a C2H2 zinc finger protein that functions as a suppressor of cell growth. This gene is often deleted or methylated and silenced in cancer cells. In addition, overexpression of this gene during fetal development is thought to be the causal factor for transient neonatal diabetes mellitus (TNDM). Alternative splicing and the use of alternative promoters results in multiple transcript variants encoding two different protein isoforms. The P1 downstream promoter of this gene is imprinted, with preferential expression from the paternal allele in many tissues. [provided by RefSeq, Nov 2015]'
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, align it with our published shRNA design sequences. If these do not align, please utilize our custom shRNA service
• Performance Guaranteed: OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
  
  For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).