PEX1 Human shRNA Lentiviral Particle (Locus ID 5189)

CAT#: TL310500V

PEX1 - Human shRNA lentiviral particles (4 unique 29mer target-specific shRNA, 1 scramble control), 0.5 ml each, >10^7 TU/ml.

Product Images

GFP signal was observed under microscope at 48 hours after transduction of TL310500A virus into HEK293 cells. TL310500A virus was prepared using lenti-shRNA TL310500A and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL310500B virus into HEK293 cells. TL310500B virus was prepared using lenti-shRNA TL310500B and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL310500C virus into HEK293 cells. TL310500C virus was prepared using lenti-shRNA TL310500C and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL310500D virus into HEK293 cells. TL310500D virus was prepared using lenti-shRNA TL310500D and TR30037 packaging kit.

Specifications

Product Data

• Locus ID: 5189
• Synonyms: HMLR1; PBD1A; PBD1B; ZWS; ZWS1
• Vector: pGFP-C-shLenti
• Format: Lentiviral particles
• RefSeq: NM_000466, NM_001282677, NM_001282678, BC035575, NM_001282678.2, NM_001282677.2
• Summary: 'This gene encodes a member of the AAA ATPase family, a large group of ATPases associated with diverse cellular activities. This protein is cytoplasmic but is often anchored to a peroxisomal membrane where it forms a heteromeric complex and plays a role in the import of proteins into peroxisomes and peroxisome biogenesis. Mutations in this gene have been associated with complementation group 1 peroxisomal disorders such as neonatal adrenoleukodystrophy, infantile Refsum disease, and Zellweger syndrome. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2013]'
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, align it with our published shRNA design sequences. If these do not align, please utilize our custom shRNA service
• Performance Guaranteed: OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
  
  For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).