NUP155 Human shRNA Lentiviral Particle (Locus ID 9631)

CAT#: TL311061V

NUP155 - Human shRNA lentiviral particles (4 unique 29mer target-specific shRNA, 1 scramble control), 0.5 ml each, >10^7 TU/ml.

Product Images

GFP signal was observed under microscope at 48 hours after transduction of TL311061A virus into HEK293 cells. TL311061A virus was prepared using lenti-shRNA TL311061A and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL311061B virus into HEK293 cells. TL311061B virus was prepared using lenti-shRNA TL311061B and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of [TL311061C] virus into HEK293 cells. [TL311061C] virus was prepared using lenti-shRNA [TL311061C] and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of [TL311061D] virus into HEK293 cells. [TL311061D] virus was prepared using lenti-shRNA [TL311061D] and TR30037 packaging kit.

Specifications

Product Data

• Locus ID: 9631
• Synonyms: ATFB15; N155
• Vector: pGFP-C-shLenti
• Format: Lentiviral particles
• RefSeq: NM_001278312, NM_004298, NM_153485, NM_004298.1, NM_004298.2, NM_004298.3, NM_153485.1, NM_153485.2, NM_001278312.1, BC039257, BC039257.1, BM478072, NM_004298.4, NM_001278312.2
• UniProt ID: O75694
• Summary: Nucleoporins are proteins that play an important role in the assembly and functioning of the nuclear pore complex (NPC) which regulates the movement of macromolecules across the nuclear envelope (NE). The protein encoded by this gene plays a role in the fusion of NE vesicles and formation of the double membrane NE. The protein may also be involved in cardiac physiology and may be associated with the pathogenesis of atrial fibrillation. Alternative splicing results in multiple transcript variants of this gene. A pseudogene associated with this gene is located on chromosome 6. [provided by RefSeq, May 2013]
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
• Performance Guaranteed: OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
  
  For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).