HSP70-1A (HSPA1A) Human shRNA Lentiviral Particle (Locus ID 3303)

CAT#: TL312308V

HSPA1A - Human shRNA lentiviral particles (4 unique 29mer target-specific shRNA, 1 scramble control), 0.5 ml each, >10^7 TU/ml.

Product Images

GFP signal was observed under microscope at 48 hours after transduction of TL312308A virus into HEK293 cells. TL312308A virus was prepared using lenti-shRNA TL312308A and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL312308B virus into HEK293 cells. TL312308B virus was prepared using lenti-shRNA TL312308B and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL312308C virus into HEK293 cells. TL312308C virus was prepared using lenti-shRNA TL312308C and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL312308D virus into HEK293 cells. TL312308D virus was prepared using lenti-shRNA TL312308D and TR30037 packaging kit.

Specifications

Product Data

• Locus ID: 3303
• Synonyms: HEL-S-103; HSP70-1; HSP70-1A; HSP70-2; HSP70.1; HSP70.2; HSP70I; HSP72; HSPA1
• Vector: pGFP-C-shLenti
• Format: Lentiviral particles
• RefSeq: NM_005345, BC002453, BC018740
• Summary: 'This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq, Jul 2008]'
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, align it with our published shRNA design sequences. If these do not align, please utilize our custom shRNA service
• Performance Guaranteed: OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
  
  For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).