HDAC3 Human shRNA Lentiviral Particle (Locus ID 8841)

CAT#: TL312494V

HDAC3 - Human shRNA lentiviral particles (4 unique 29mer target-specific shRNA, 1 scramble control), 0.5 ml each, >10^7 TU/ml.

Product Images

GFP signal was observed under microscope at 48 hours after transduction of TL312494A virus into HEK293 cells. TL312494A virus was prepared using lenti-shRNA TL312494A and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL312494B virus into HEK293 cells. TL312494B virus was prepared using lenti-shRNA TL312494B and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL312494C virus into HEK293 cells. TL312494C virus was prepared using lenti-shRNA TL312494C and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL312494D virus into HEK293 cells. TL312494D virus was prepared using lenti-shRNA TL312494D and TR30037 packaging kit.

Specifications

Product Data

• Locus ID: 8841
• Synonyms: HD3; KDAC3; RPD3; RPD3-2
• Vector: pGFP-C-shLenti
• Format: Lentiviral particles
• RefSeq: NM_003883, NM_001355039, NM_001355040, NM_001355041, NR_149164, NR_149165, NR_149166, NR_149167, NR_149168, NR_149169, BC000614, BM461585
• Summary: Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene. [provided by RefSeq, Jul 2008]
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, align it with our published shRNA design sequences. If these do not align, please utilize our custom shRNA service
• Performance Guaranteed: OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
  
  For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).