Hyaluronan synthase 2 (HAS2) Human shRNA Lentiviral Particle (Locus ID 3037)

CAT#: TL312518V

HAS2 - Human shRNA lentiviral particles (4 unique 29mer target-specific shRNA, 1 scramble control), 0.5 ml each, >10^7 TU/ml.

Product Images

GFP signal was observed under microscope at 48 hours after transduction of TL312518A virus into HEK293 cells. TL312518A virus was prepared using lenti-shRNA TL312518A and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL312518B virus into HEK293 cells. TL312518B virus was prepared using lenti-shRNA TL312518B and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of [TL312518C] virus into HEK293 cells. [TL312518C] virus was prepared using lenti-shRNA [TL312518C] and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of [TL312518D] virus into HEK293 cells. [TL312518D] virus was prepared using lenti-shRNA [TL312518D] and TR30037 packaging kit.

Specifications

Product Data

• Locus ID: 3037
• Vector: pGFP-C-shLenti
• Format: Lentiviral particles
• RefSeq: NM_005328, NM_005328.1, NM_005328.2, BC069353, BC109071, BC109072
• UniProt ID: Q92819
• Summary: Hyaluronan or hyaluronic acid (HA) is a high molecular weight unbranched polysaccharide synthesized by a wide variety of organisms from bacteria to mammals, and is a constituent of the extracellular matrix. It consists of alternating glucuronic acid and N-acetylglucosamine residues that are linked by beta-1-3 and beta-1-4 glycosidic bonds. HA is synthesized by membrane-bound synthase at the inner surface of the plasma membrane, and the chains are extruded through pore-like structures into the extracellular space. It serves a variety of functions, including space filling, lubrication of joints, and provision of a matrix through which cells can migrate. HA is actively produced during wound healing and tissue repair to provide a framework for ingrowth of blood vessels and fibroblasts. Changes in the serum concentration of HA are associated with inflammatory and degenerative arthropathies such as rheumatoid arthritis. In addition, the interaction of HA with the leukocyte receptor CD44 is important in tissue-specific homing by leukocytes, and overexpression of HA receptors has been correlated with tumor metastasis. HAS2 is a member of the newly identified vertebrate gene family encoding putative hyaluronan synthases, and its amino acid sequence shows significant homology to glycosaminoglycan synthetase (DG42) from Xenopus laevis, and human and murine hyaluronan synthase 1. [provided by RefSeq, Jul 2008]
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
• Performance Guaranteed: OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
  
  For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).