FUCA1 Human shRNA Lentiviral Particle (Locus ID 2517)

CAT#: TL312904V

FUCA1 - Human shRNA lentiviral particles (4 unique 29mer target-specific shRNA, 1 scramble control), 0.5 ml each, >10^7 TU/ml.

Product Images

GFP signal was observed under microscope at 48 hours after transduction of TL312904A virus into HEK293 cells. TL312904A virus was prepared using lenti-shRNA TL312904A and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL312904B virus into HEK293 cells. TL312904B virus was prepared using lenti-shRNA TL312904B and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL312904C virus into HEK293 cells. TL312904C virus was prepared using lenti-shRNA TL312904C and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL312904D virus into HEK293 cells. TL312904D virus was prepared using lenti-shRNA TL312904D and TR30037 packaging kit.

Specifications

Product Data

• Locus ID: 2517
• Synonyms: FUCA
• Vector: pGFP-C-shLenti
• Format: Lentiviral particles
• RefSeq: NM_000147, BC017338
• Summary: 'The protein encoded by this gene is a lysosomal enzyme involved in the degradation of fucose-containing glycoproteins and glycolipids. Mutations in this gene are associated with fucosidosis (FUCA1D), which is an autosomal recessive lysosomal storage disease. A pseudogene of this locus is present on chr 2.[provided by RefSeq, Oct 2009]'
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, align it with our published shRNA design sequences. If these do not align, please utilize our custom shRNA service
• Performance Guaranteed: OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
  
  For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).