PDE5A Human shRNA Lentiviral Particle (Locus ID 8654)

CAT#: TL316826V

PDE5A - Human shRNA lentiviral particles (4 unique 29mer target-specific shRNA, 1 scramble control), 0.5 ml each, >10^7 TU/ml.

Product Images

GFP signal was observed under microscope at 48 hours after transduction of TL316826A virus into HEK293 cells. TL316826A virus was prepared using lenti-shRNA TL316826A and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL316826B virus into HEK293 cells. TL316826B virus was prepared using lenti-shRNA TL316826B and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL316826C virus into HEK293 cells. TL316826C virus was prepared using lenti-shRNA TL316826C and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL316826D virus into HEK293 cells. TL316826D virus was prepared using lenti-shRNA TL316826D and TR30037 packaging kit.

Specifications

Product Data

• Locus ID: 8654
• Synonyms: CGB-PDE; CN5A; PDE5
• Vector: pGFP-C-shLenti
• Format: Lentiviral particles
• RefSeq: NM_001083, NM_033430, NM_033431, NM_033437, BC126233, BM920622, BM978332
• Summary: This gene encodes a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Alternative splicing of this gene results in three transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, align it with our published shRNA design sequences. If these do not align, please utilize our custom shRNA service
• Performance Guaranteed: OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
  
  For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).