CD177 Human shRNA Lentiviral Particle (Locus ID 57126)

CAT#: TL317350V

CD177 - Human shRNA lentiviral particles (4 unique 29mer target-specific shRNA, 1 scramble control), 0.5 ml each, >10^7 TU/ml.

Product Images

GFP signal was observed under microscope at 48 hours after transduction of TL317350A virus into HEK293 cells. TL317350A virus was prepared using lenti-shRNA TL317350A and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL317350B virus into HEK293 cells. TL317350B virus was prepared using lenti-shRNA TL317350B and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL317350C virus into HEK293 cells. TL317350C virus was prepared using lenti-shRNA TL317350C and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL317350D virus into HEK293 cells. TL317350D virus was prepared using lenti-shRNA TL317350D and TR30037 packaging kit.

Specifications

Product Data

• Locus ID: 57126
• Synonyms: HNA-2a; HNA2A; NB1; NB1 GP; PRV-1; PRV1
• Vector: pGFP-C-shLenti
• Format: Lentiviral particles
• RefSeq: NM_020406, BC029167
• Summary: This gene encodes a glycosyl-phosphatidylinositol (GPI)-linked cell surface glycoprotein that plays a role in neutrophil activation. The protein can bind platelet endothelial cell adhesion molecule-1 and function in neutrophil transmigration. Mutations in this gene are associated with myeloproliferative diseases. Over-expression of this gene has been found in patients with polycythemia rubra vera. Autoantibodies against the protein may result in pulmonary transfusion reactions, and it may be involved in Wegener's granulomatosis. A related pseudogene, which is adjacent to this gene on chromosome 19, has been identified. [provided by RefSeq, Apr 2014]
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, align it with our published shRNA design sequences. If these do not align, please utilize our custom shRNA service
• Performance Guaranteed: OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
  
  For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).