JAK2 Human shRNA Lentiviral Particle (Locus ID 3717)

CAT#: TL320395V

JAK2 - Human shRNA lentiviral particles (4 unique 29mer target-specific shRNA, 1 scramble control), 0.5 ml each, >10^7 TU/ml.

Product Images

GFP signal was observed under microscope at 48 hours after transduction of TL320395A virus into HEK293 cells. TL320395A virus was prepared using lenti-shRNA TL320395A and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL320395B virus into HEK293 cells. TL320395B virus was prepared using lenti-shRNA TL320395B and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL320395C virus into HEK293 cells. TL320395C virus was prepared using lenti-shRNA TL320395C and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL320395D virus into HEK293 cells. TL320395D virus was prepared using lenti-shRNA TL320395D and TR30037 packaging kit.

Specifications

Product Data

• Locus ID: 3717
• Synonyms: JTK10; THCYT3
• Vector: pGFP-C-shLenti
• Format: Lentiviral particles
• RefSeq: NM_001322198, NM_001322199, NM_004972, NM_001322194, NM_001322195, NM_001322196, NM_001322204, BC039695, BC043187
• Summary: 'This gene encodes a non-receptor tyrosine kinase that plays a central role in cytokine and growth factor signalling. The primary isoform of this protein has an N-terminal FERM domain that is required for erythropoietin receptor association, an SH2 domain that binds STAT transcription factors, a pseudokinase domain and a C-terminal tyrosine kinase domain. Cytokine binding induces autophosphorylation and activation of this kinase. This kinase then recruits and phosphorylates signal transducer and activator of transcription (STAT) proteins. Growth factors like TGF-beta 1 also induce phosphorylation and activation of this kinase and translocation of downstream STAT proteins to the nucleus where they influence gene transcription. Mutations in this gene are associated with numerous inflammatory diseases and malignancies. This gene is a downstream target of the pleiotropic cytokine IL6 that is produced by B cells, T cells, dendritic cells and macrophages to produce an immune response or inflammation. Disregulation of the IL6/JAK2/STAT3 signalling pathways produces increased cellular proliferation and myeloproliferative neoplasms of hematopoietic stem cells. A nonsynonymous mutation in the pseudokinase domain of this gene disrupts the domains inhibitory effect and results in constitutive tyrosine phosphorylation activity and hypersensitivity to cytokine signalling. This gene and the IL6/JAK2/STAT3 signalling pathway is a therapeutic target for the treatment of excessive inflammatory responses to viral infections. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2020]'
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, align it with our published shRNA design sequences. If these do not align, please utilize our custom shRNA service
• Performance Guaranteed: OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
  
  For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).