Fkbp5 Mouse shRNA Lentiviral Particle (Locus ID 14229)

CAT#: TL500712V

Fkbp5 - Mouse shRNA lentiviral particles (4 unique 29mer target-specific shRNA, 1 scramble control), 0.5 ml each, >10^7 TU/ml.

Product Images

GFP signal was observed under microscope at 48 hours after transduction of TL500712A virus into HEK293 cells. TL500712A virus was prepared using lenti-shRNA TL500712A and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL500712B virus into HEK293 cells. TL500712B virus was prepared using lenti-shRNA TL500712B and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL500712C virus into HEK293 cells. TL500712C virus was prepared using lenti-shRNA TL500712C and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL500712D virus into HEK293 cells. TL500712D virus was prepared using lenti-shRNA TL500712D and TR30037 packaging kit.

Specifications

Product Data

• Locus ID: 14229
• Synonyms: D17Ertd592e; Dit1; FKBP-5; FKBP51
• Vector: pGFP-C-shLenti
• Format: Lentiviral particles
• RefSeq: BC015260, NM_010220
• Summary: Immunophilin protein with PPIase and co-chaperone activities. Component of unligated steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). Plays a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors maintaining the complex into the cytoplasm when unliganded. [UniProtKB/Swiss-Prot Function]
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, align it with our published shRNA design sequences. If these do not align, please utilize our custom shRNA service
• Performance Guaranteed: OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
  
  For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).