Zdhhc3 Mouse shRNA Lentiviral Particle (Locus ID 69035)

CAT#: TL504254V

Zdhhc3 - Mouse shRNA lentiviral particles (4 unique 29mer target-specific shRNA, 1 scramble control), 0.5 ml each, >10^7 TU/ml.

Product Images

GFP signal was observed under microscope at 48 hours after transduction of TL504254A virus into HEK293 cells. TL504254A virus was prepared using lenti-shRNA TL504254A and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL504254B virus into HEK293 cells. TL504254B virus was prepared using lenti-shRNA TL504254B and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of [TL504254C] virus into HEK293 cells. [TL504254C] virus was prepared using lenti-shRNA [TL504254C] and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of [TL504254D] virus into HEK293 cells. [TL504254D] virus was prepared using lenti-shRNA [TL504254D] and TR30037 packaging kit.

Specifications

Product Data

• Locus ID: 69035
• Synonyms: 1110020O22Rik; 1810006O10Rik; 2210017C02Rik; DHHC-3; GODZ; Gramp1; Zfp373
• Vector: pGFP-C-shLenti
• Format: Lentiviral particles
• RefSeq: BC025126, BC052464, NM_026917, NM_026917.1, NM_026917.2, NM_026917.3, NM_026917.4, NM_026917.5, BC005531, BC018334, BM963754, NM_026917.6
• UniProt ID: Q8R173
• Summary: Palmitoyltransferase with broad specificity. Palmitoylates GABA receptors on their gamma subunit (GABRG1, GABRG2 and GABRG3), which regulates synaptic clustering and/or cell surface stability (PubMed:15229235). Palmitoylates glutamate receptors GRIA1 and GRIA2, which leads to their retention in Golgi (PubMed:16129400). May also palmitoylate DLG4, DNAJC5 and SNAP25 (PubMed:15603741, PubMed:25253725).[UniProtKB/Swiss-Prot Function]
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
• Performance Guaranteed: OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
  
  For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).