Prkaca Mouse shRNA Lentiviral Particle (Locus ID 18747)

CAT#: TL510604V

Prkaca - Mouse shRNA lentiviral particles (4 unique 29mer target-specific shRNA, 1 scramble control), 0.5 ml each, >10^7 TU/ml.

Product Images

GFP signal was observed under microscope at 48 hours after transduction of TL510604A virus into HEK293 cells. TL510604A virus was prepared using lenti-shRNA TL510604A and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of [TL510604C] virus into HEK293 cells. [TL510604C] virus was prepared using lenti-shRNA [TL510604C] and TR30037 packaging kit.

Specifications

Product Data

• Locus ID: 18747
• Synonyms: C; P; Pk; Pkaca; PKCD
• Vector: pGFP-C-shLenti
• Format: Lentiviral particles
• RefSeq: BC003238, BC054834, NM_001277898, NM_008854, NM_008854.1, NM_008854.2, NM_008854.3, NM_008854.4, NM_008854.5, NM_001277898.1
• UniProt ID: P05132
• Summary: This gene encodes a member of the serine/threonine protein kinase family. The holoenzyme, protein kinase A (also known as cyclic-AMP dependent protein kinase), mediates cellular response to changes in cyclic-AMP levels. This gene encodes the alpha catalytic subunit of protein kinase A. Protein kinase A-mediated signaling is transduced via phosphorylation of target proteins, and is important for many cellular functions, including mammalian sperm maturation and motility. Alternative splicing results in multiple transcript variants. A pseudogene of this gene has been defined on the X chromosome. [provided by RefSeq, Apr 2013]
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
• Performance Guaranteed: OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
  
  For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).