Trim11 Mouse shRNA Lentiviral Particle (Locus ID 94091)

CAT#: TL512492V

Trim11 - Mouse shRNA lentiviral particles (4 unique 29mer target-specific shRNA, 1 scramble control), 0.5 ml each, >10^7 TU/ml.

Product Images

GFP signal was observed under microscope at 48 hours after transduction of TL512492A virus into HEK293 cells. TL512492A virus was prepared using lenti-shRNA TL512492A and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL512492B virus into HEK293 cells. TL512492B virus was prepared using lenti-shRNA TL512492B and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of [TL512492C] virus into HEK293 cells. [TL512492C] virus was prepared using lenti-shRNA [TL512492C] and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of [TL512492D] virus into HEK293 cells. [TL512492D] virus was prepared using lenti-shRNA [TL512492D] and TR30037 packaging kit.

Specifications

Product Data

• Locus ID: 94091
• Vector: pGFP-C-shLenti
• Format: Lentiviral particles
• RefSeq: BC020102, NM_001290988, NM_053168, NM_053168.1, NM_053168.2, NM_001290988.1, BC020102.1
• UniProt ID: Q99PQ2
• Summary: E3 ubiquitin-protein ligase that promotes the degradation of insoluble ubiquitinated proteins, including insoluble PAX6, poly-Gln repeat expanded HTT and poly-Ala repeat expanded ARX. Mediates PAX6 ubiquitination leading to proteasomal degradation, thereby modulating cortical neurogenesis. May also inhibit PAX6 transcriptional activity, possibly in part by preventing the binding of PAX6 to its consensus sequences. May contribute to the regulation of the intracellular level of HN (humanin) or HN-containing proteins through the proteasomal degradation pathway. Mediates MED15 ubiquitination leading to proteasomal degradation. May contribute to the innate restriction of retroviruses. Upon overexpression, reduces HIV-1 and murine leukemia virus infectivity, by suppressing viral gene expression. Antiviral activity depends on a functional E3 ubiquitin-protein ligase domain. May regulate TRIM5 turnover via the proteasome pathway, thus counteracting the TRIM5-mediated cross-species restriction of retroviral infection at early stages of the retroviral life cycle.[UniProtKB/Swiss-Prot Function]
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
• Performance Guaranteed: OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
  
  For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).