FGFR3 Human shRNA Plasmid Kit (Locus ID 2261)
CAT#: TF320355
FGFR3 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector
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Specifications
Product Data | |
Locus ID | 2261 |
Synonyms | ACH; CD333; CEK2; HSFGFR3EX; JTK4 |
Vector | pRFP-C-RS |
E. coli Selection | Chloramphenicol |
Mammalian Cell Selection | Puromycin |
Format | Retroviral plasmids |
Kit Components | FGFR3 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector(Gene ID = 2261). 5µg purified plasmid DNA per constructNon-effective 29-mer scrambled shRNA cassette in pRFP-C-RS Vector, TR30015, included for free. |
RefSeq | NM_000142, NM_001163213, NM_022965, NM_001354809, NM_001354810, NR_148971, BC121175, BC128610, BC153824, BC166684 |
Summary | 'This gene encodes a member of the fibroblast growth factor receptor (FGFR) family, with its amino acid sequence being highly conserved between members and among divergent species. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member binds acidic and basic fibroblast growth hormone and plays a role in bone development and maintenance. Mutations in this gene lead to craniosynostosis and multiple types of skeletal dysplasia. [provided by RefSeq, Aug 2017]' |
shRNA Design | These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, align it with our published shRNA design sequences. If these do not align, please utilize our custom shRNA service |
Performance Guaranteed | OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred). |
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