Meg3 Mouse shRNA Plasmid (Locus ID 17263)

CAT#: TG501330

Meg3 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector

Specifications

Product Data

• Locus ID: 17263
• Synonyms: Meg3, R74756, R75394, AI425946, AW108224, D12Bwg1266e, 2900016C05Rik, 3110050O07Rik, 6330408G06Rik
• Vector: pGFP-V-RS
• Format: Retroviral plasmids
• Kit Components: Meg3 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector(Gene ID = 17263). 5µg purified plasmid DNA per construct
  Non-effective 29-mer scrambled shRNA cassette in pGFP-V-RS Vector, TR30013, included for free.
• RefSeq: BC048191, NM_144513, NR_003633, NR_027651, NR_027652, NR_046475, NR_104450, NR_104451, BC024818, BC028971, BC051667, BC055835, BC094421
• Summary: This gene is paternally-imprinted and thus expressed predominantly from the maternal allele. It generates alternatively-spliced long non protein-coding RNAs that overlap with the microRNAs Mir770 and Mir1906-1. Transcripts from this gene function as negative regulators of growth and may be down-regulated in tumor cells. This gene is expressed in a reciprocal pattern from the nearby Dlk1 (delta-like 1 homolog) gene, which is paternally-expressed. Deletion of this locus disrupts imprinting in this genomic region and results in perinatal morbidity. [provided by RefSeq, Dec 2013]
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, align it with our published shRNA design sequences. If these do not align, please utilize our custom shRNA service
• Performance Guaranteed: OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
  
  For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).